The Vibrio cholerae Quorum-Sensing Protein VqmA Integrates Cell Density, Environmental, and Host-Derived Cues into the Control of Virulence.

TitleThe Vibrio cholerae Quorum-Sensing Protein VqmA Integrates Cell Density, Environmental, and Host-Derived Cues into the Control of Virulence.
Publication TypeJournal Article
Year of Publication2020
AuthorsMashruwala, AA, Bassler, BL
JournalmBio
Volume11
Issue4
Date Published2020 07 28
ISSN2150-7511
KeywordsAnaerobiosis, Bacterial Proteins, Bile Acids and Salts, Biofilms, Gene Expression Regulation, Bacterial, Host-Pathogen Interactions, Humans, Quorum Sensing, Signal Transduction, Vibrio cholerae, Virulence
Abstract

<p>Quorum sensing is a chemical communication process in which bacteria use the production, release, and detection of signal molecules called autoinducers to orchestrate collective behaviors. The human pathogen requires quorum sensing to infect the small intestine. There, encounters the absence of oxygen and the presence of bile salts. We show that these two stimuli differentially affect quorum-sensing function and, in turn, pathogenicity. First, during anaerobic growth, does not produce the CAI-1 autoinducer, while it continues to produce the DPO autoinducer, suggesting that CAI-1 may encode information specific to the aerobic lifestyle of Second, the quorum-sensing receptor-transcription factor called VqmA, which detects the DPO autoinducer, also detects the lack of oxygen and the presence of bile salts. Detection occurs via oxygen-, bile salt-, and redox-responsive disulfide bonds that alter VqmA DNA binding activity. We propose that VqmA serves as an information processing hub that integrates quorum-sensing information, redox status, the presence or absence of oxygen, and host cues. In response to the information acquired through this mechanism, appropriately modulates its virulence output. Quorum sensing (QS) is a process of chemical communication that bacteria use to orchestrate collective behaviors. QS communication relies on chemical signal molecules called autoinducers. QS regulates virulence in , the causative agent of the disease cholera. Transit into the human small intestine, the site of cholera infection, exposes to the host environment. In this study, we show that the combination of two stimuli encountered in the small intestine, the absence of oxygen and the presence of host-produced bile salts, impinge on QS function and, in turn, pathogenicity. We suggest that possessing a QS system that is responsive to multiple environmental, host, and cell density cues enables to fine-tune its virulence capacity in the human intestine.</p>

DOI10.1128/mBio.01572-20
Alternate JournalmBio
PubMed ID32723922
PubMed Central IDPMC7387800
Grant ListR37 GM065859 / GM / NIGMS NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States