Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos. Author Hongtao Chen, Thomas Gregor Publication Year 2020 Type Journal Article Abstract Elucidating the biological implications of higher order chromatin architectures in animal development requires simultaneous, quantitative measurements of chromatin dynamics and transcriptional activity in living specimen. Here we describe a multicolor labeling and live imaging approach in embryos of the fruit fly Drosophila melanogaster. The method allows simultaneous measurement of movements of specific loci and their transcriptional activity for developmental genes, enabling new approaches to probe the interaction between 3D chromatin architecture and regulation of gene expression in development. Keywords Animals, Drosophila Proteins, Transcription, Genetic, RNA, Green Fluorescent Proteins, Animals, Genetically Modified, Female, Male, Drosophila melanogaster, Gene Expression Regulation, Developmental, Microscopy, Confocal, Genetic Loci, Chromatin, CRISPR-Cas Systems, Fluorescent Dyes, Gene Editing, Color, Gene Knock-In Techniques Journal Methods Mol Biol Volume 2166 Pages 373-384 Date Published 2020 ISSN Number 1940-6029 DOI 10.1007/978-1-0716-0712-1_22 Alternate Journal Methods Mol Biol PMCID PMC8130451 PMID 32710421 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML