| Title | Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos. |
| Publication Type | Journal Article |
| Year of Publication | 2020 |
| Authors | Chen, H, Gregor, T |
| Journal | Methods Mol Biol |
| Volume | 2166 |
| Pagination | 373-384 |
| Date Published | 2020 |
| ISSN | 1940-6029 |
| Keywords | Animals, Animals, Genetically Modified, Chromatin, Color, CRISPR-Cas Systems, Drosophila melanogaster, Drosophila Proteins, Female, Fluorescent Dyes, Gene Editing, Gene Expression Regulation, Developmental, Gene Knock-In Techniques, Genetic Loci, Green Fluorescent Proteins, Male, Microscopy, Confocal, RNA, Transcription, Genetic |
| Abstract | <p>Elucidating the biological implications of higher order chromatin architectures in animal development requires simultaneous, quantitative measurements of chromatin dynamics and transcriptional activity in living specimen. Here we describe a multicolor labeling and live imaging approach in embryos of the fruit fly Drosophila melanogaster. The method allows simultaneous measurement of movements of specific loci and their transcriptional activity for developmental genes, enabling new approaches to probe the interaction between 3D chromatin architecture and regulation of gene expression in development.</p> |
| DOI | 10.1007/978-1-0716-0712-1_22 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 32710421 |
| PubMed Central ID | PMC8130451 |
| Grant List | R01 GM097275 / GM / NIGMS NIH HHS / United States U01 DA047730 / DA / NIDA NIH HHS / United States U01 DK127429 / DK / NIDDK NIH HHS / United States U01 EB021239 / EB / NIBIB NIH HHS / United States |
