Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos.

TitleUsing RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos.
Publication TypeJournal Article
Year of Publication2020
AuthorsChen, H, Gregor, T
JournalMethods Mol Biol
Volume2166
Pagination373-384
Date Published2020
ISSN1940-6029
KeywordsAnimals, Animals, Genetically Modified, Chromatin, Color, CRISPR-Cas Systems, Drosophila melanogaster, Drosophila Proteins, Female, Fluorescent Dyes, Gene Editing, Gene Expression Regulation, Developmental, Gene Knock-In Techniques, Genetic Loci, Green Fluorescent Proteins, Male, Microscopy, Confocal, RNA, Transcription, Genetic
Abstract

<p>Elucidating the biological implications of higher order chromatin architectures in animal development requires simultaneous, quantitative measurements of chromatin dynamics and transcriptional activity in living specimen. Here we describe a multicolor labeling and live imaging approach in embryos of the fruit fly Drosophila melanogaster. The method allows simultaneous measurement of movements of specific loci and their transcriptional activity for developmental genes, enabling new approaches to probe the interaction between 3D chromatin architecture and regulation of gene expression in development.</p>

DOI10.1007/978-1-0716-0712-1_22
Alternate JournalMethods Mol Biol
PubMed ID32710421
PubMed Central IDPMC8130451
Grant ListR01 GM097275 / GM / NIGMS NIH HHS / United States
U01 DA047730 / DA / NIDA NIH HHS / United States
U01 DK127429 / DK / NIDDK NIH HHS / United States
U01 EB021239 / EB / NIBIB NIH HHS / United States