Uncoupling neurogenic gene networks in the embryo.

TitleUncoupling neurogenic gene networks in the embryo.
Publication TypeJournal Article
Year of Publication2017
AuthorsRogers, WA, Goyal, Y, Yamaya, K, Shvartsman, SY, Levine, MS
JournalGenes Dev
Date Published2017 04 01
KeywordsAnimals, Cell Lineage, Cells, Cultured, Central Nervous System, CRISPR-Cas Systems, Drosophila, Drosophila Proteins, Embryo, Nonmammalian, Epidermal Growth Factor, ErbB Receptors, Female, Gene Expression Regulation, Developmental, Gene Regulatory Networks, Male, Membrane Proteins, Neurogenesis, Receptors, Invertebrate Peptide, Signal Transduction

<p>The EGF signaling pathway specifies neuronal identities in the embryo by regulating developmental patterning genes such as (). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.</p>

Alternate JournalGenes Dev
PubMed ID28428262
PubMed Central IDPMC5411704
Grant ListR01 GM086537 / GM / NIGMS NIH HHS / United States
R35 GM118147 / GM / NIGMS NIH HHS / United States