|Title||Uncoupling neurogenic gene networks in the Drosophila embryo.|
|Publication Type||Journal Article|
|Year of Publication||2017|
|Authors||Rogers, WA, Goyal, Y, Yamaya, K, Shvartsman, SY, Levine, MS|
|Date Published||2017 Apr 20|
The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neurons defective (ind). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.
|Alternate Journal||Genes Dev.|