Title | A tumor-specific endogenous repetitive element is induced by herpesviruses. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Nogalski, MT, Solovyov, A, Kulkarni, AS, Desai, N, Oberstein, A, Levine, AJ, Ting, DT, Shenk, T, Greenbaum, BD |
Journal | Nat Commun |
Volume | 10 |
Issue | 1 |
Pagination | 90 |
Date Published | 2019 01 09 |
ISSN | 2041-1723 |
Keywords | Cell Line, Cell Movement, Fibroblasts, Gene Expression Regulation, Herpesviridae, Human Genetics, Humans, In Situ Hybridization, Interspersed Repetitive Sequences, RNA, Up-Regulation |
Abstract | Tandem satellite repeats account for 3% of the human genome. One of them, Human Satellite II (HSATII), is highly expressed in several epithelial cancers and cancer cell lines. Here we report an acute induction of HSATII RNA in human cells infected with two herpes viruses. We show that human cytomegalovirus (HCMV) IE1 and IE2 proteins cooperate to induce HSATII RNA affecting several aspects of the HCMV replication cycle, viral titers and infected-cell processes. HSATII RNA expression in tissue from two chronic HCMV colitis patients correlates with the strength of CMV antigen staining. Thus, endogenous HSATII RNA synthesis after herpesvirus infections appears to have functionally important consequences for viral replication and may provide a novel insight into viral pathogenesis. The HSATII induction seen in both infected and cancer cells suggests possible convergence upon common HSATII-based regulatory mechanisms in these seemingly disparate diseases. |
DOI | 10.1038/s41467-018-07944-x |
Alternate Journal | Nat Commun |
PubMed ID | 30626867 |
PubMed Central ID | PMC6327058 |
Grant List | 1P30CA196521-01 / NH / NIH HHS / United States R01 AI081848 / AI / NIAID NIH HHS / United States 7R01AI081848-04 / NH / NIH HHS / United States AI112951 / NH / NIH HHS / United States R56 AI112951 / AI / NIAID NIH HHS / United States R01 AI112951 / AI / NIAID NIH HHS / United States P30 CA196521 / CA / NCI NIH HHS / United States |