A TRUSTED targeted mass spectrometry assay for pan-herpesvirus protein detection.

TitleA TRUSTED targeted mass spectrometry assay for pan-herpesvirus protein detection.
Publication TypeJournal Article
Year of Publication2022
AuthorsKennedy, MA, Tyl, MD, Betsinger, CN, Federspiel, JD, Sheng, X, Arbuckle, JH, Kristie, TM, Cristea, IM
JournalCell Rep
Date Published2022 May 10
KeywordsCytomegalovirus, Herpesvirus 1, Human, Herpesvirus 8, Human, Humans, Mass Spectrometry, Virus Replication

<p>The presence and abundance of viral proteins within host cells are part of the essential signatures of the cellular stages of viral infections. However, methods that can comprehensively detect and quantify these proteins are still limited, particularly for viruses with large protein coding capacity. Here, we design and experimentally validate a mass spectrometry-based Targeted herpesviRUS proTEin Detection (TRUSTED) assay for monitoring human viruses representing the three Herpesviridae subfamilies-herpes simplex virus type 1, human cytomegalovirus (HCMV), and Kaposi sarcoma-associated herpesvirus. We demonstrate assay applicability for (1) capturing the temporal cascades of viral replication, (2) detecting proteins throughout a range of virus concentrations and in in vivo models of infection, (3) assessing the effects of clinical therapeutic agents and sirtuin-modulating compounds, (4) studies using different laboratory and clinical viral strains, and (5) discovering a role for carbamoyl phosphate synthetase 1 in supporting HCMV replication.</p>

Alternate JournalCell Rep
PubMed ID35545036
PubMed Central IDPMC9245836
Grant ListF31 AI154796 / AI / NIAID NIH HHS / United States
R01 GM114141 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States