A Transport Model for Estimating the Time Course of ERK Activation in the C. elegans Germline.

TitleA Transport Model for Estimating the Time Course of ERK Activation in the C. elegans Germline.
Publication TypeJournal Article
Year of Publication2015
AuthorsMattingly, HH, Chen, JJ, Arur, S, Shvartsman, SY
JournalBiophys J
Volume109
Issue11
Pagination2436-45
Date Published2015 Dec 01
ISSN1542-0086
KeywordsAnimals, Caenorhabditis elegans, Enzyme Activation, Extracellular Signal-Regulated MAP Kinases, Germ Cells, Kinetics, MAP Kinase Signaling System, Models, Biological, Protein Transport
Abstract

<p>The Caenorhabditis elegans germline is a well-studied model system for investigating the control of cell fate by signaling pathways. Cell signals at the distal tip of the germline promote cell proliferation; just before the loop, signals couple cell maturation to organism-level nutrient status; at the proximal end of the germline, signals coordinate oocyte maturation and fertilization in the presence of sperm. The latter two events require dual phosphorylation and activation of ERK, the effector molecule of the Ras/MAPK cascade. In C. elegans, ERK is known as MPK-1. At this point, none of today's methods for real-time monitoring of dually phosphorylated MPK-1 are working in the germline. Consequently, quantitative understanding of the MPK-1-dependent processes during germline development is limited. Here, we make a step toward advancing this understanding using a model-based framework that reconstructs the time course of MPK-1 activation from a snapshot of a fixed germline. Our approach builds on a number of recent studies for estimating temporal dynamics from fixed organisms, but takes advantage of the anatomy of the germline to simplify the analysis. Our model predicts that the MPK-1 signal turns on ∼30 h into germ cell progression and peaks ∼7 h later. </p>

DOI10.1016/j.bpj.2015.10.021
Alternate JournalBiophys. J.
PubMed ID26636953
PubMed Central IDPMC4675862
Grant ListR01 GM086537 / GM / NIGMS NIH HHS / United States
R01 GM098200 / GM / NIGMS NIH HHS / United States
GM 98200 / GM / NIGMS NIH HHS / United States
R01GM086537 / GM / NIGMS NIH HHS / United States