|Title||Transcriptome analysis of adult Caenorhabditis elegans cells reveals tissue-specific gene and isoform expression.|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Kaletsky, R, Yao, V, Williams, A, Runnels, AM, Tadych, A, Zhou, S, Troyanskaya, OG, Murphy, CT|
|Date Published||2018 Aug 10|
The biology and behavior of adults differ substantially from those of developing animals, and cell-specific information is critical for deciphering the biology of multicellular animals. Thus, adult tissue-specific transcriptomic data are critical for understanding molecular mechanisms that control their phenotypes. We used adult cell-specific isolation to identify the transcriptomes of C. elegans' four major tissues (or "tissue-ome"), identifying ubiquitously expressed and tissue-specific "enriched" genes. These data newly reveal the hypodermis' metabolic character, suggest potential worm-human tissue orthologies, and identify tissue-specific changes in the Insulin/IGF-1 signaling pathway. Tissue-specific alternative splicing analysis identified a large set of collagen isoforms. Finally, we developed a machine learning-based prediction tool for 76 sub-tissue cell types, which we used to predict cellular expression differences in IIS/FOXO signaling, stage-specific TGF-β activity, and basal vs. memory-induced CREB transcription. Together, these data provide a rich resource for understanding the biology governing multicellular adult animals.
|Alternate Journal||PLoS Genet.|