Transcriptional Regulation Contributes to Prioritized Detoxification of Hydrogen Peroxide over Nitric Oxide. Author Kristin Adolfsen, Wen Chou, Mark Brynildsen Publication Year 2019 Type Journal Article Abstract Hydrogen peroxide (HO) and nitric oxide (NO·) are toxic metabolites that immune cells use to attack pathogens. These antimicrobials can be present at the same time in phagosomes, and it remains unclear how bacteria deal with these insults when simultaneously present. Here, using , we observed that simultaneous exposure to HO and NO· leads to prioritized detoxification, where enzymatic removal of NO· is impeded until HO has been eliminated. This phenomenon is reminiscent of carbon catabolite repression (CCR), where preferred carbon sources are catabolized prior to less desirable substrates; however, HO and NO· are toxic, growth-inhibitory compounds rather than growth-promoting nutrients. To understand how NO· detoxification is delayed by HO whereas HO detoxification proceeds unimpeded, we confirmed that the effect depended on Hmp, which is the main NO· detoxification enzyme, and used an approach that integrated computational modeling and experimentation to delineate and test potential mechanisms. Plausible interactions included HO-dependent inhibition of transcription and translation, direct inhibition of Hmp catalysis, and competition for reducing equivalents between Hmp and HO-degrading enzymes. Experiments illustrated that Hmp catalysis and NAD(P)H supply were not impaired by HO, whereas transcription and translation were diminished. A dependence of this phenomenon on transcriptional regulation parallels CCR, and we found it to involve the transcriptional repressor NsrR. Collectively, these data suggest that bacterial regulation of growth inhibitor detoxification has similarities to the regulation of growth substrate consumption, which could have ramifications for infectious disease, bioremediation, and biocatalysis from inhibitor-containing feedstocks. Bacteria can be exposed to HO and NO· concurrently within phagosomes. In such multistress situations, bacteria could have evolved to simultaneously degrade both toxic metabolites or preferentially detoxify one over the other. Here, we found that simultaneous exposure to HO and NO· leads to prioritized detoxification, where detoxification of NO· is hampered until HO has been eliminated. This phenomenon resembles CCR, where bacteria consume one substrate over others in carbon source mixtures. Further experimentation revealed a central role for transcriptional regulation in the prioritization of HO over NO·, which is also important to CCR. This study suggests that regulatory scenarios observed in bacterial consumption of growth-promoting compound mixtures can be conserved in bacterial detoxification of toxic metabolite mixtures. Keywords Gene Expression Regulation, Bacterial, Escherichia coli, Transcription, Genetic, Escherichia coli Proteins, Nitric Oxide, Hydrogen Peroxide, Oxidative Stress, Inactivation, Metabolic Journal J Bacteriol Volume 201 Issue 14 Date Published 2019 Jul 15 ISSN Number 1098-5530 DOI 10.1128/JB.00081-19 Alternate Journal J Bacteriol PMCID PMC6597392 PMID 31061166 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML