Title | Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Kaletsky, R, Murphy, CT |
Journal | Methods Mol Biol |
Volume | 2144 |
Pagination | 177-186 |
Date Published | 2020 |
ISSN | 1940-6029 |
Keywords | Aging, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Profiling, Longevity, Mutation, Phenotype, Receptor, Insulin, Signal Transduction |
Abstract | <p>Multicellular organisms are composed of distinct cells and tissues that coordinate highly orchestrated responses to environmental challenges, including those that arise with age. Since C. elegans is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe a method for the FACS-based isolation and RNA sequencing of dissociated cells from adult C. elegans. This technique is amenable to profiling the cell- and tissue-specific gene expression changes in C. elegans mutants, including aging models, such as the daf-2/insulin-like signaling (IIS) pathway, and in wild-type animals with age.</p> |
DOI | 10.1007/978-1-0716-0592-9_16 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 32410035 |
Grant List | DP1 GM119167 / GM / NIGMS NIH HHS / United States R01 AG034446 / AG / NIA NIH HHS / United States |