Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age.

TitleTranscriptional Profiling of C. elegans Adult Cells and Tissues with Age.
Publication TypeJournal Article
Year of Publication2020
AuthorsKaletsky, R, Murphy, CT
JournalMethods Mol Biol
Date Published2020
KeywordsAging, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Profiling, Longevity, Mutation, Phenotype, Receptor, Insulin, Signal Transduction

<p>Multicellular organisms are composed of distinct cells and tissues that coordinate highly orchestrated responses to environmental challenges, including those that arise with age. Since C. elegans is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe a method for the FACS-based isolation and RNA sequencing of dissociated cells from adult C. elegans. This technique is amenable to profiling the cell- and tissue-specific gene expression changes in C. elegans mutants, including aging models, such as the daf-2/insulin-like signaling (IIS) pathway, and in wild-type animals with age.</p>

Alternate JournalMethods Mol Biol
PubMed ID32410035
Grant ListR01 AG034446 / AG / NIA NIH HHS / United States
DP1 GM119167 / GM / NIGMS NIH HHS / United States