A tps1Δ persister-like state in Saccharomyces cerevisiae is regulated by MKT1.

TitleA tps1Δ persister-like state in Saccharomyces cerevisiae is regulated by MKT1.
Publication TypeJournal Article
Year of Publication2020
AuthorsGibney, PA, Chen, A, Schieler, A, Chen, JC, Xu, Y, Hendrickson, DG, R McIsaac, S, Rabinowitz, JD, Botstein, D
JournalPLoS One
Volume15
Issue5
Paginatione0233779
Date Published2020
ISSN1932-6203
KeywordsElectron Transport Complex III, Fermentation, Fructose, Glucose, Glucosyltransferases, Loss of Function Mutation, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Trehalose
Abstract

<p>Trehalose metabolism in yeast has been linked to a variety of phenotypes, including heat resistance, desiccation tolerance, carbon-source utilization, and sporulation. The relationships among the several phenotypes of mutants unable to synthesize trehalose are not understood, even though the pathway is highly conserved. One of these phenotypes is that tps1Δ strains cannot reportedly grow on media containing glucose or fructose, even when another carbon source they can use (e.g. galactose) is present. Here we corroborate the recent observation that a small fraction of yeast tps1Δ cells do grow on glucose, unlike the majority of the population. This is not due to a genetic alteration, but instead resembles the persister phenotype documented in many microorganisms and cancer cells undergoing lethal stress. We extend these observations to show that this phenomenon is glucose-specific, as it does not occur on another highly fermented carbon source, fructose. We further demonstrate that this phenomenon appears to be related to mitochondrial complex III function, but unrelated to inorganic phosphate levels in the cell, as had previously been suggested. Finally, we found that this phenomenon is specific to S288C-derived strains, and is the consequence of a variant in the MKT1 gene.</p>

DOI10.1371/journal.pone.0233779
Alternate JournalPLoS ONE
PubMed ID32470059
PubMed Central IDPMC7259636