Title | Systems-level analysis of mechanisms regulating yeast metabolic flux. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Hackett, SR, Zanotelli, VRT, Xu, W, Goya, J, Park, JO, Perlman, DH, Gibney, PA, Botstein, D, Storey, JD, Rabinowitz, JD |
Journal | Science |
Volume | 354 |
Issue | 6311 |
Date Published | 2016 Oct 28 |
ISSN | 1095-9203 |
Keywords | Allosteric Regulation, Citrates, Glycolysis, Kinetics, Metabolic Networks and Pathways, Nitrogen, Pyruvate Kinase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins |
Abstract | <p>Cellular metabolic fluxes are determined by enzyme activities and metabolite abundances. Biochemical approaches reveal the impact of specific substrates or regulators on enzyme kinetics but do not capture the extent to which metabolite and enzyme concentrations vary across physiological states and, therefore, how cellular reactions are regulated. We measured enzyme and metabolite concentrations and metabolic fluxes across 25 steady-state yeast cultures. We then assessed the extent to which flux can be explained by a Michaelis-Menten relationship between enzyme, substrate, product, and potential regulator concentrations. This revealed three previously unrecognized instances of cross-pathway regulation, which we biochemically verified. One of these involved inhibition of pyruvate kinase by citrate, which accumulated and thereby curtailed glycolytic outflow in nitrogen-limited yeast. Overall, substrate concentrations were the strongest driver of the net rates of cellular metabolic reactions, with metabolite concentrations collectively having more than double the physiological impact of enzymes.</p> |
DOI | 10.1126/science.aaf2786 |
Alternate Journal | Science |
PubMed ID | 27789812 |
PubMed Central ID | PMC5414049 |
Grant List | R01 GM046406 / GM / NIGMS NIH HHS / United States R01 HG002913 / HG / NHGRI NIH HHS / United States R37 GM046406 / GM / NIGMS NIH HHS / United States P50 GM071508 / GM / NIGMS NIH HHS / United States P30 CA016359 / CA / NCI NIH HHS / United States R01 CA163591 / CA / NCI NIH HHS / United States |