Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.

TitleSystematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.
Publication TypeJournal Article
Year of Publication2022
AuthorsFauser, F, Vilarrasa-Blasi, J, Onishi, M, Ramundo, S, Patena, W, Millican, M, Osaki, J, Philp, C, Nemeth, M, Salomé, PA, Li, X, Wakao, S, Kim, RG, Kaye, Y, Grossman, AR, Niyogi, KK, Merchant, SS, Cutler, SR, Walter, P, Dinneny, JR, Jonikas, MC, Jinkerson, RE
JournalNat Genet
Volume54
Issue5
Pagination705-714
Date Published2022 05
ISSN1546-1718
KeywordsArabidopsis, Chlamydomonas reinhardtii, Eukaryota, Phenotype, Photosynthesis
Abstract

<p>Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined the phenotypes of more than 58,000 mutants under more than 121 different environmental growth conditions and chemical treatments. A total of 59% of genes are represented by at least one mutant that showed a phenotype, providing clues to the functions of thousands of genes. Mutant phenotypic profiles place uncharacterized genes into functional pathways such as DNA repair, photosynthesis, the CO-concentrating mechanism and ciliogenesis. We illustrate the value of this resource by validating phenotypes and gene functions, including three new components of an actin cytoskeleton defense pathway. The data also inform phenotype discovery in land plants; mutants in Arabidopsis thaliana genes exhibit phenotypes similar to those we observed in their Chlamydomonas homologs. We anticipate that this resource will guide the functional characterization of genes across the tree of life.</p>

DOI10.1038/s41588-022-01052-9
Alternate JournalNat Genet
PubMed ID35513725
PubMed Central IDPMC9110296
Grant ListDP2 GM119137 / GM / NIGMS NIH HHS / United States
55108535 / HHMI / Howard Hughes Medical Institute / United States