Surface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development.

TitleSurface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development.
Publication TypeJournal Article
Year of Publication2017
AuthorsKim, MKevin, Zhao, A, Wang, A, Brown, ZZ, Muir, TW, Stone, HA, Bassler, BL
JournalNat Microbiol
Volume2
Pagination17080
Date Published2017 May 22
ISSN2058-5276
KeywordsBiofilms, Environmental Microbiology, Quorum Sensing, Second Messenger Systems, Staphylococcus aureus, Surface Properties
Abstract

<p>Bacteria use a process called quorum sensing to communicate and orchestrate collective behaviours, including virulence factor secretion and biofilm formation. Quorum sensing relies on the production, release, accumulation and population-wide detection of signal molecules called autoinducers. Here, we develop concepts to coat surfaces with quorum-sensing-manipulation molecules as a method to control collective behaviours. We probe this strategy using Staphylococcus aureus. Pro- and anti-quorum-sensing molecules can be covalently attached to surfaces using click chemistry, where they retain their abilities to influence bacterial behaviours. We investigate key features of the compounds, linkers and surfaces necessary to appropriately position molecules to interact with cognate receptors and the ability of modified surfaces to resist long-term storage, repeated infections, host plasma components and flow-generated stresses. Our studies highlight how this surface approach can be used to make colonization-resistant materials against S. aureus and other pathogens and how the approach can be adapted to promote beneficial behaviours of bacteria on surfaces.</p>

DOI10.1038/nmicrobiol.2017.80
Alternate JournalNat Microbiol
PubMed ID28530651
PubMed Central IDPMC5526357
Grant List / / Howard Hughes Medical Institute / United States
R01 AI042783 / AI / NIAID NIH HHS / United States
R01 GM065859 / GM / NIGMS NIH HHS / United States
R37 GM065859 / GM / NIGMS NIH HHS / United States