A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry.

TitleA Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry.
Publication TypeJournal Article
Year of Publication2018
AuthorsStadlmeier, M, Bogena, J, Wallner, M, Wühr, M, Carell, T
JournalAngew Chem Int Ed Engl
Volume57
Issue11
Pagination2958-2962
Date Published2018 Mar 05
ISSN1521-3773
Abstract

Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes provides multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. Here we report a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.

DOI10.1002/anie.201708867
Alternate JournalAngew. Chem. Int. Ed. Engl.
PubMed ID29316131