Subcellular proteomics.

TitleSubcellular proteomics.
Publication TypeJournal Article
Year of Publication2021
AuthorsChristopher, JA, Stadler, C, Martin, CE, Morgenstern, M, Pan, Y, Betsinger, CN, Rattray, DG, Mahdessian, D, Gingras, A-C, Warscheid, B, Lehtiö, J, Cristea, IM, Foster, LJ, Emili, A, Lilley, KS
JournalNat Rev Methods Primers
Volume1
Date Published2021/04/29
ISSN2662-8449
Abstract

The eukaryotic cell is compartmentalized into subcellular niches, including membrane-bound and membrane-less organelles. Proteins localize to these niches to fulfil their function, enabling discreet biological processes to occur in synchrony. Dynamic movement of proteins between niches is essential for cellular processes such as signalling, growth, proliferation, motility and programmed cell death, and mutations causing aberrant protein localization are associated with a wide range of diseases. Determining the location of proteins in different cell states and cell types and how proteins relocalize following perturbation is important for understanding their functions, related cellular processes and pathologies associated with their mislocalization. In this Primer, we cover the major spatial proteomics methods for determining the location, distribution and abundance of proteins within subcellular structures. These technologies include fluorescent imaging, protein proximity labelling, organelle purification and cell-wide biochemical fractionation. We describe their workflows, data outputs and applications in exploring different cell biological scenarios, and discuss their main limitations. Finally, we describe emerging technologies and identify areas that require technological innovation to allow better characterization of the spatial proteome.

DOI10.1038/s43586-021-00029-y
Alternate JournalNat Rev Methods Primers
PubMed ID34549195
PubMed Central IDPMC8451152
Grant ListR01 AG064932 / AG / NIA NIH HHS / United States
R01 GM114141 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States
R01 DK110520 / DK / NIDDK NIH HHS / United States
R01 HD089275 / HD / NICHD NIH HHS / United States
RF1 AG061706 / AG / NIA NIH HHS / United States
UL1 TR001430 / TR / NCATS NIH HHS / United States