Title | Stability and nuclear localization of yeast telomerase depend on protein components of RNase P/MRP. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | P Garcia, D, Leach, RW, Wadsworth, GM, Choudhary, K, Li, H, Aviran, S, Kim, HD, Zakian, VA |
Journal | Nat Commun |
Volume | 11 |
Issue | 1 |
Pagination | 2173 |
Date Published | 2020 May 01 |
ISSN | 2041-1723 |
Keywords | Cell Nucleus, DNA-Binding Proteins, Methylation, Protein Binding, Ribonuclease P, Ribonucleoproteins, RNA, RNA 3' End Processing, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Telomerase, Telomere |
Abstract | <p>RNase P and MRP are highly conserved, multi-protein/RNA complexes with essential roles in processing ribosomal and tRNAs. Three proteins found in both complexes, Pop1, Pop6, and Pop7 are also telomerase-associated. Here, we determine how temperature sensitive POP1 and POP6 alleles affect yeast telomerase. At permissive temperatures, mutant Pop1/6 have little or no effect on cell growth, global protein levels, the abundance of Est1 and Est2 (telomerase proteins), and the processing of TLC1 (telomerase RNA). However, in pop mutants, TLC1 is more abundant, telomeres are short, and TLC1 accumulates in the cytoplasm. Although Est1/2 binding to TLC1 occurs at normal levels, Est1 (and hence Est3) binding is highly unstable. We propose that Pop-mediated stabilization of Est1 binding to TLC1 is a pre-requisite for formation and nuclear localization of the telomerase holoenzyme. Furthermore, Pop proteins affect TLC1 and the RNA subunits of RNase P/MRP in very different ways.</p> |
DOI | 10.1038/s41467-020-15875-9 |
Alternate Journal | Nat Commun |
PubMed ID | 32358529 |
PubMed Central ID | PMC7195438 |
Grant List | R01 GM112882 / GM / NIGMS NIH HHS / United States |