Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.

TitleStability, affinity, and chromatic variants of the glutamate sensor iGluSnFR.
Publication TypeJournal Article
Year of Publication2018
AuthorsMarvin, JS, Scholl, B, Wilson, DE, Podgorski, K, Kazemipour, A, Müller, JAlexander, Schoch, S, Quiroz, FJosé Urra, Rebola, N, Bao, H, Little, JP, Tkachuk, AN, Cai, E, Hantman, AW, Wang, SS-H, DePiero, VJ, Borghuis, BG, Chapman, ER, Dietrich, D, DiGregorio, DA, Fitzpatrick, D, Looger, LL
JournalNat Methods
Date Published2018 Nov
KeywordsAnimals, Color, Female, Ferrets, Fluorescent Dyes, Glutamic Acid, Green Fluorescent Proteins, Male, Mice, Inbred C57BL, Microscopy, Fluorescence, Neurons, Retina, Visual Cortex

<p>Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.</p>

Alternate JournalNat Methods
PubMed ID30377363
PubMed Central IDPMC6394230
Grant List / HHMI / Howard Hughes Medical Institute / United States