Title | Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Marvin, JS, Scholl, B, Wilson, DE, Podgorski, K, Kazemipour, A, Müller, JAlexander, Schoch, S, Quiroz, FJosé Urra, Rebola, N, Bao, H, Little, JP, Tkachuk, AN, Cai, E, Hantman, AW, Wang, SS-H, DePiero, VJ, Borghuis, BG, Chapman, ER, Dietrich, D, DiGregorio, DA, Fitzpatrick, D, Looger, LL |
Journal | Nat Methods |
Volume | 15 |
Issue | 11 |
Pagination | 936-939 |
Date Published | 2018 Nov |
ISSN | 1548-7105 |
Keywords | Animals, Color, Female, Ferrets, Fluorescent Dyes, Glutamic Acid, Green Fluorescent Proteins, Male, Mice, Inbred C57BL, Microscopy, Fluorescence, Neurons, Retina, Visual Cortex |
Abstract | <p>Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.</p> |
DOI | 10.1038/s41592-018-0171-3 |
Alternate Journal | Nat Methods |
PubMed ID | 30377363 |
PubMed Central ID | PMC6394230 |
Grant List | / HHMI / Howard Hughes Medical Institute / United States |