sp. ATCC 55076 harbours the largest actinomycete chromosome to date and the kistamicin biosynthetic gene cluster.

Titlesp. ATCC 55076 harbours the largest actinomycete chromosome to date and the kistamicin biosynthetic gene cluster.
Publication TypeJournal Article
Year of Publication2017
AuthorsNazari, B, Forneris, CC, Gibson, MI, Moon, K, Schramma, KR, Seyedsayamdost, MR
JournalMedchemcomm
Volume8
Issue4
Pagination780-788
Date Published2017 Apr 01
ISSN2040-2503
Abstract

Glycopeptide antibiotics (GPAs) have served as potent clinical drugs and as an inspiration to chemists in various disciplines. Among known GPAs, complestatin, chloropeptin, and kistamicin are unique in that they contain an unusual indole-phenol crosslink. The mechanism of formation of this linkage is unknown, and to date, the biosynthetic gene cluster of only one GPA with an indole-phenol crosslink, that of complestatin, has been identified. Here, we report the genome sequence of the kistamicin producersp. ATCC 55076. We find that this strain harbours the largest actinobacterial chromosome to date, consisting of a single linear chromosome of ∼13.1 Mbp. AntiSMASH analysis shows that ∼32 biosynthetic gene clusters and ∼10% of the genome are devoted to production of secondary metabolites, which include 1,6-dihydroxyphenazine and nomuricin, a new anthraquinone-type pentacyclic compound that we report herein. The kistamicin gene cluster () was identified bioinformatically. A unique feature ofis that it contains two cytochrome P450 enzymes, which likely catalyze three crosslinking reactions. These findings set the stage for examining the biosynthesis of kistamicin and its unusual indole-phenol crosslink in the future.

DOI10.1039/c6md00637j
Alternate JournalMedchemcomm
PubMed ID28626548
PubMed Central IDPMC5463735
Grant ListR00 GM098299 / GM / NIGMS NIH HHS / United States