Size scaling in collective cell growth.

TitleSize scaling in collective cell growth.
Publication TypeJournal Article
Year of Publication2021
AuthorsDiegmiller, R, Doherty, CA, Stern, T, Alsous, JImran, Shvartsman, SY
JournalDevelopment
Volume148
Issue18
Date Published2021 09 15
ISSN1477-9129
KeywordsAnimals, Biological Evolution, Cell Proliferation, Developmental Biology, Diptera, Germ Cells, Oogenesis, Organelles
Abstract

<p>Size is a fundamental feature of living entities and is intimately tied to their function. Scaling laws, which can be traced to D'Arcy Thompson and Julian Huxley, have emerged as a powerful tool for studying regulation of the growth dynamics of organisms and their constituent parts. Yet, throughout the 20th century, as scaling laws were established for single cells, quantitative studies of the coordinated growth of multicellular structures have lagged, largely owing to technical challenges associated with imaging and image processing. Here, we present a supervised learning approach for quantifying the growth dynamics of germline cysts during oogenesis. Our analysis uncovers growth patterns induced by the groupwise developmental dynamics among connected cells, and differential growth rates of their organelles. We also identify inter-organelle volumetric scaling laws, finding that nurse cell growth is linear over several orders of magnitude. Our approach leverages the ever-increasing quantity and quality of imaging data, and is readily amenable for studies of collective cell growth in other developmental contexts, including early mammalian embryogenesis and germline development.</p>

DOI10.1242/dev.199663
Alternate JournalDevelopment
PubMed ID34463760
PubMed Central IDPMC8489028
Grant ListR01 GM134204 / GM / NIGMS NIH HHS / United States
F31 HD098835 / HD / NICHD NIH HHS / United States