Single mRNA Molecule Detection in Drosophila.

TitleSingle mRNA Molecule Detection in Drosophila.
Publication TypeJournal Article
Year of Publication2018
AuthorsLittle, SC, Gregor, T
JournalMethods Mol Biol
Volume1649
Pagination127-142
Date Published2018
ISSN1940-6029
Abstract

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.

DOI10.1007/978-1-4939-7213-5_8
Alternate JournalMethods Mol. Biol.
PubMed ID29130194