Single mRNA Molecule Detection in Drosophila.

TitleSingle mRNA Molecule Detection in Drosophila.
Publication TypeJournal Article
Year of Publication2018
AuthorsLittle, SC, Gregor, T
JournalMethods Mol Biol
Date Published2018
KeywordsAnimals, Chromatography, High Pressure Liquid, Drosophila melanogaster, Embryo, Nonmammalian, Fluorescent Dyes, Formaldehyde, In Situ Hybridization, Fluorescence, Microscopy, Confocal, Oligonucleotide Probes, Polymers, RNA, Messenger, Tissue Fixation

<p>Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.</p>

Alternate JournalMethods Mol Biol
PubMed ID29130194
PubMed Central IDPMC5772605
Grant ListR01 GM097275 / GM / NIGMS NIH HHS / United States
U01 DA047730 / DA / NIDA NIH HHS / United States