| Title | Single mRNA Molecule Detection in Drosophila. |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Little, SC, Gregor, T |
| Journal | Methods Mol Biol |
| Volume | 1649 |
| Pagination | 127-142 |
| Date Published | 2018 |
| ISSN | 1940-6029 |
| Keywords | Animals, Chromatography, High Pressure Liquid, Drosophila melanogaster, Embryo, Nonmammalian, Fluorescent Dyes, Formaldehyde, In Situ Hybridization, Fluorescence, Microscopy, Confocal, Oligonucleotide Probes, Polymers, RNA, Messenger, Tissue Fixation |
| Abstract | <p>Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.</p> |
| DOI | 10.1007/978-1-4939-7213-5_8 |
| Alternate Journal | Methods Mol Biol |
| PubMed ID | 29130194 |
| PubMed Central ID | PMC5772605 |
| Grant List | R01 GM097275 / GM / NIGMS NIH HHS / United States U01 DA047730 / DA / NIDA NIH HHS / United States |
