Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields.

TitleSimultaneous cellular-resolution optical perturbation and imaging of place cell firing fields.
Publication TypeJournal Article
Year of Publication2014
AuthorsRickgauer, JPeter, Deisseroth, K, Tank, DW
JournalNat Neurosci
Volume17
Issue12
Pagination1816-24
Date Published2014 Dec
ISSN1546-1726
KeywordsAnimals, Hippocampus, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurons, Optogenetics, Photic Stimulation, Space Perception
Abstract

<p>Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, λ = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, λ = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields. </p>

DOI10.1038/nn.3866
Alternate JournalNat. Neurosci.
PubMed ID25402854
PubMed Central IDPMC4459599
Grant ListP50-GM071508 / GM / NIGMS NIH HHS / United States
R01 MH075957 / MH / NIMH NIH HHS / United States
R01-MH083686 / MH / NIMH NIH HHS / United States
R01 MH099647 / MH / NIMH NIH HHS / United States
R01 MH083686 / MH / NIMH NIH HHS / United States
U01 NS090541 / NS / NINDS NIH HHS / United States
R37 DA035377 / DA / NIDA NIH HHS / United States
R01 DA035377 / DA / NIDA NIH HHS / United States