simMSG: an experimental design tool for high-throughput genotyping of hybrids.

TitlesimMSG: an experimental design tool for high-throughput genotyping of hybrids.
Publication TypeJournal Article
Year of Publication2016
AuthorsSchumer, M, Cui, R, Rosenthal, GG, Andolfatto, P
JournalMol Ecol Resour
Volume16
Issue1
Pagination183-92
Date Published2016 01 16
ISSN1755-0998
KeywordsChimera, Computer Simulation, Genotype, Genotyping Techniques, High-Throughput Nucleotide Sequencing, Hybridization, Genetic
Abstract

Hybridization between closely related species, whether naturally occurring or laboratory generated, is a useful tool for mapping the genetic basis of the phenotypic traits that distinguish species. The development of next-generation sequencing techniques has greatly improved our ability to assign ancestry to hybrid genomes. One such next-generation sequencing technique, multiplexed shotgun genotyping (or MSG), can be a powerful tool for genotyping hybrids. However, it is difficult a priori to predict the accuracy of MSG in natural hybrids because accuracy depends on ancestry tract length and number of ancestry informative markers. Here, we present a simulator, 'simMSG', that will allow researchers to design MSG experiments and show that in many cases MSG can accurately assign ancestry to hundreds of thousands of sites in the genomes of natural hybrids. The simMSG tool can be used to design experiments for diverse applications including QTL mapping, genotyping introgressed lines or admixture mapping.

DOI10.1111/1755-0998.12434
Alternate JournalMol Ecol Resour
PubMed ID26032857