Title | Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Trcek, T, Douglas, TE, Grosch, M, Yin, Y, Eagle, WVI, Gavis, ER, Shroff, H, Rothenberg, E, Lehmann, R |
Journal | Mol Cell |
Volume | 78 |
Issue | 5 |
Pagination | 941-950.e12 |
Date Published | 2020 Jun 04 |
ISSN | 1097-4164 |
Keywords | Animals, Cytoplasmic Granules, Drosophila melanogaster, Drosophila Proteins, Nuclear Proteins, Organelles, Ribonucleoproteins, RNA, RNA Transport, RNA, Messenger |
Abstract | <p>mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism by which multiple mRNAs derived from the same gene assemble into discrete homotypic clusters. We demonstrate that in vivo mRNA localization to granules and self-assembly within granules are governed by different mRNA features: localization is encoded by specific RNA regions, whereas self-assembly involves the entire mRNA, does not involve sequence-specific, ordered intermolecular RNA:RNA interactions, and is thus RNA sequence independent. We propose that the ability of mRNAs to self-sort into homotypic assemblies is an inherent property of an messenger ribonucleoprotein (mRNP) that is augmented under conditions that increase RNA concentration, such as upon enrichment in RNA-protein granules, a process that appears conserved in diverse cellular contexts and organisms.</p> |
DOI | 10.1016/j.molcel.2020.05.008 |
Alternate Journal | Mol Cell |
PubMed ID | 32464092 |
PubMed Central ID | PMC7325742 |
Grant List | K99 HD088675 / HD / NICHD NIH HHS / United States R35 GM126967 / GM / NIGMS NIH HHS / United States R01 GM067758 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States R00 HD088675 / HD / NICHD NIH HHS / United States R37 HD041900 / HD / NICHD NIH HHS / United States |