SARS-CoV-2 receptor networks in diabetic kidney disease, BK-Virus nephropathy and COVID-19 associated acute kidney injury.

TitleSARS-CoV-2 receptor networks in diabetic kidney disease, BK-Virus nephropathy and COVID-19 associated acute kidney injury.
Publication TypeJournal Article
Year of Publication2020
AuthorsMenon, R, Otto, EA, Sealfon, R, Nair, V, Wong, AK, Theesfeld, CL, Chen, X, Wang, Y, Boppanna, A, Luo, J, Yang, Y, Kasson, PM, Schaub, JA, Berthier, CC, Eddy, S, Lienczewski, CC, Godfrey, B, Dagenais, SL, Sohaney, R, Hartman, J, Fermin, D, Subramanian, L, Looker, HC, Harder, JL, Mariani, LH, Hodgin, JB, Sexton, JZ, Wobus, CE, Naik, AS, Nelson, RG, Troyanskaya, OG, Kretzler, M
JournalmedRxiv
Date Published2020 May 13
Abstract

<p>COVID-19 morbidity and mortality is significantly increased in patients with diabetes and kidney disease via unknown mechanisms. SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2) for entry into human host cells, and ACE2 levels in target cells may influence SARS-CoV-2 susceptibility. We investigated how pre-existing conditions and drug treatments alter receptor expression in kidney tissue. Using single cell RNA profiling (scRNAseq) to assess ACE2 and associated SARS-CoV-2 proteases in healthy living donors (LD) kidneys, diabetic kidney disease (DKD), and in kidney injury during viral infection, ACE2 expression was primarily associated with proximal tubular epithelial cells (PTEC). ACE2 mRNA expression levels were significantly upregulated in DKD versus LD, however, ACE2 levels were not altered by exposures to renin angiotensin aldosterone system (RAAS) inhibitors. ACE2+ expression signatures were defined by differential expression analysis and characterized by Bayesian integrative analysis of a large compendium of public -omics datasets, resulting in the identification of network modules induced in ACE2 positive PTEC in DKD and BK virus nephropathy. These ACE2 upregulated cell programs were linked to viral entry, immune activation, endomembrane reorganization, and RNA processing and overlapped significantly with the cellular responses induced by SARS-CoV-2 infection. Similar cellular programs were activated in ACE2-positive PTEC isolated in a urine sample from a COVID19 patient with acute kidney injury, suggesting a consistent ACE2-coregulated expression program that may interact with SARS-Cov-2 infection processes. The SARS-CoV-2 receptor associated gene signatures could seed further research into therapeutic strategies for COVID-19. Functional networks of gene expression signatures are available for further exploration to researchers at HumanBase (hb.flatironinstitute.org/covid-kidney).</p>

DOI10.1101/2020.05.09.20096511
Alternate JournalmedRxiv
PubMed ID32511461
PubMed Central IDPMC7241118
Grant ListUH3 DK114907 / DK / NIDDK NIH HHS / United States
P30 DK092926 / DK / NIDDK NIH HHS / United States
UH3 DK114923 / DK / NIDDK NIH HHS / United States
UH3 DK114908 / DK / NIDDK NIH HHS / United States
U2C DK114886 / DK / NIDDK NIH HHS / United States
UH3 DK114915 / DK / NIDDK NIH HHS / United States
UH3 DK114861 / DK / NIDDK NIH HHS / United States
P60 DK020572 / DK / NIDDK NIH HHS / United States
UH3 DK114866 / DK / NIDDK NIH HHS / United States
UH3 DK114870 / DK / NIDDK NIH HHS / United States
P30 DK020572 / DK / NIDDK NIH HHS / United States
P30 DK081943 / DK / NIDDK NIH HHS / United States
UH3 DK114933 / DK / NIDDK NIH HHS / United States
Z01 DK069062 / ImNIH / Intramural NIH HHS / United States
U54 DK083912 / DK / NIDDK NIH HHS / United States
UH3 DK114920 / DK / NIDDK NIH HHS / United States
R24 DK082841 / DK / NIDDK NIH HHS / United States
UH3 DK114926 / DK / NIDDK NIH HHS / United States
UH3 DK114937 / DK / NIDDK NIH HHS / United States