A Review on Quantitative Multiplexed Proteomics.

TitleA Review on Quantitative Multiplexed Proteomics.
Publication TypeJournal Article
Year of Publication2019
AuthorsPappireddi, N, Martin, L, Wühr, M
JournalChembiochem
Volume20
Issue10
Pagination1210-1224
Date Published2019 05 15
ISSN1439-7633
KeywordsHumans, Isotope Labeling, Proteins, Proteome, Proteomics, Tandem Mass Spectrometry
Abstract

<p>Over the last few decades, mass spectrometry-based proteomics has become an increasingly powerful tool that is now able to routinely detect and quantify thousands of proteins. A major advance for global protein quantification was the introduction of isobaric tags, which, in a single experiment, enabled the global quantification of proteins across multiple samples. Herein, these methods are referred to as multiplexed proteomics. The principles, advantages, and drawbacks of various multiplexed proteomics techniques are discussed and compared with alternative approaches. We also discuss how the emerging combination of multiplexing with targeted proteomics might enable the reliable and high-quality quantification of very low abundance proteins across multiple conditions. Lastly, we suggest that fusing multiplexed proteomics with data-independent acquisition approaches might enable the comparison of hundreds of different samples without missing values, while maintaining the superb measurement precision and accuracy obtainable with isobaric tag quantification.</p>

DOI10.1002/cbic.201800650
Alternate JournalChembiochem
PubMed ID30609196
PubMed Central IDPMC6520187
Grant ListT32 GM007388 / GM / NIGMS NIH HHS / United States
R35 GM128813 / GM / NIGMS NIH HHS / United States