Revealing epithelial morphogenetic mechanisms through live imaging.

TitleRevealing epithelial morphogenetic mechanisms through live imaging.
Publication TypeJournal Article
Year of Publication2022
AuthorsFarahani, PE, Nelson, CM
JournalCurr Opin Genet Dev
Volume72
Pagination61-68
Date Published2022 Feb
ISSN1879-0380
KeywordsMechanical Phenomena, Morphogenesis
Abstract

<p>Epithelial morphogenesis is guided by mechanical forces and biochemical signals that vary spatiotemporally. As many morphogenetic events are driven by rapid cellular processes, understanding morphogenesis requires monitoring development in real time. Here, we discuss how live-imaging approaches can help identify morphogenetic mechanisms otherwise missed in static snapshots of development. We begin with a summary of live-imaging strategies, including recent advances that push the limits of spatiotemporal resolution and specimen size. We then describe recent efforts that employ live imaging to uncover morphogenetic mechanisms. We conclude by discussing how information collected from live imaging can be enhanced by genetically encoded biosensors and spatiotemporal perturbation techniques to determine the dynamics of patterning of developmental signals and their importance for guiding morphogenesis.</p>

DOI10.1016/j.gde.2021.10.007
Alternate JournalCurr Opin Genet Dev
PubMed ID34864332
PubMed Central IDPMC8860867
Grant ListR01 HD099030 / HD / NICHD NIH HHS / United States
R01 HL120142 / HL / NHLBI NIH HHS / United States
U01 CA214292 / CA / NCI NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States