Representative subsampling of sedimenting blood.

TitleRepresentative subsampling of sedimenting blood.
Publication TypeJournal Article
Year of Publication2019
AuthorsRallabandi, B, Nunes, JK, Perazzo, A, Gershtein, S, Stone, HA
JournalProc Math Phys Eng Sci
Volume475
Issue2227
Pagination20190223
Date Published2019 Jul
ISSN1364-5021
Abstract

It is often necessary to extract a small amount of a suspension, such as blood, from a larger sample of the same material for the purposes of diagnostics, testing or imaging. A practical challenge is that the cells in blood sediment noticeably on the time scale of a few minutes, making a representative subsampling of the original sample challenging. Guided by experimental data, we develop a Kynch sedimentation model to discuss design considerations that ensure a representative subsampling of blood, from a container of constant cross-sectional area, for the entire range of physiologically relevant hematocrit over a specified time of interest. Additionally, we show that this design may be modified to exploit the sedimentation and perform subsampling to achieve either higher or lower hematocrit relative to that of the original sample. Thus, our method provides a simple tool to either concentrate or dilute small quantities of blood or other sedimenting suspensions.

DOI10.1098/rspa.2019.0223
Alternate JournalProc. Math. Phys. Eng. Sci.
PubMed ID31423102
PubMed Central IDPMC6694302