On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.

TitleOn the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
Publication TypeJournal Article
Year of Publication2015
AuthorsPeshkin, L, Wühr, M, Pearl, E, Haas, W, Freeman, RM, Gerhart, JC, Klein, AM, Horb, M, Gygi, SP, Kirschner, MW
JournalDev Cell
Volume35
Issue3
Pagination383-94
Date Published2015 Nov 09
ISSN1878-1551
KeywordsAging, Animals, Embryonic Development, Gene Expression Regulation, Developmental, Protein Biosynthesis, Proteome, RNA, Messenger, Transcription, Genetic, Xenopus laevis, Xenopus Proteins
Abstract

<p>A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10,000 proteins and ∼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome. </p>

DOI10.1016/j.devcel.2015.10.010
Alternate JournalDev. Cell
PubMed ID26555057
PubMed Central IDPMC4776761
Grant ListP40OD010997 / OD / NIH HHS / United States
R01 HD073104 / HD / NICHD NIH HHS / United States
R01 DK077197 / DK / NIDDK NIH HHS / United States
R01 GM103785 / GM / NIGMS NIH HHS / United States
R01HD073104 / HD / NICHD NIH HHS / United States
P40 OD010997 / OD / NIH HHS / United States
R01GM103785 / GM / NIGMS NIH HHS / United States