The recombinant expression systems for structure determination of eukaryotic membrane proteins.

TitleThe recombinant expression systems for structure determination of eukaryotic membrane proteins.
Publication TypeJournal Article
Year of Publication2014
AuthorsHe, Y, Wang, K, Yan, N
JournalProtein Cell
Volume5
Issue9
Pagination658-72
Date Published2014 Sep
ISSN1674-8018
KeywordsAnimals, Escherichia coli, Eukaryotic Cells, Genetic Vectors, HEK293 Cells, Humans, Insecta, Membrane Proteins, Recombinant Proteins, Yeasts
Abstract

Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.

DOI10.1007/s13238-014-0086-4
Alternate JournalProtein Cell
PubMed ID25119489
PubMed Central IDPMC4145085
Grant List / / Howard Hughes Medical Institute / United States