|Title||Radical SAM Enzyme QmpB Installs Two 9-Membered Ring Sactionine Macrocycles during Biogenesis of a Ribosomal Peptide Natural Product.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Caruso, A, Seyedsayamdost, MR|
|Journal||J Org Chem|
We report the reaction catalyzed by QmpB, a new radical -adenosylmethionine enzyme encoded by a ribosomal peptide natural product gene cluster in . Using isotopic labeling, site-directed mutagenesis, high-resolution mass spectrometry, and multidimensional NMR spectroscopy, we show that QmpB installs two 9-membered ring sactionine bridges, connecting a Cys residue with an upstream Asn via an α-thioether bridge, with the two macrocycles separated by a single residue. QmpB is only the second type II sactionine synthase characterized to date.
|Alternate Journal||J Org Chem|