Quorum sensing in Escherichia coli and Salmonella typhimurium.

TitleQuorum sensing in Escherichia coli and Salmonella typhimurium.
Publication TypeJournal Article
Year of Publication1998
AuthorsSurette, MG, Bassler, BL
JournalProc Natl Acad Sci U S A
Volume95
Issue12
Pagination7046-50
Date Published1998 Jun 09
ISSN0027-8424
KeywordsBiological Assay, Biosensing Techniques, Cell Communication, Cell Count, Escherichia coli, Salmonella typhimurium, Signal Transduction
Abstract

<p>Escherichia coli and Salmonella typhimurium strains grown in Luria-Bertani medium containing glucose secrete a small soluble heat labile organic molecule that is involved in intercellular communication. The factor is not produced when the strains are grown in Luria-Bertani medium in the absence of glucose. Maximal secretion of the substance occurs in midexponential phase, and the extracellular activity is degraded as the glucose is depleted from the medium or by the onset of stationary phase. Destruction of the signaling molecule in stationary phase indicates that, in contrast to other quorum-sensing systems, quorum sensing in E. coli and S. typhimurium is critical for regulating behavior in the prestationary phase of growth. Our results further suggest that the signaling factor produced by E. coli and S. typhimurium is used to communicate both the cell density and the metabolic potential of the environment. Several laboratory and clinical strains of E. coli and S. typhimurium were screened for production of the signaling molecule, and most strains make it under conditions similar to those shown here for E. coli AB1157 and S. typhimurium LT2. However, we also show that E. coli strain DH5alpha does not make the soluble factor, indicating that this highly domesticated strain has lost the gene(s) or biosynthetic machinery necessary to produce the signaling substance. Implications for the involvement of quorum sensing in pathogenesis are discussed.</p>

DOI10.1073/pnas.95.12.7046
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID9618536
PubMed Central IDPMC22733