Quorum sensing controls biofilm formation in Vibrio cholerae.

TitleQuorum sensing controls biofilm formation in Vibrio cholerae.
Publication TypeJournal Article
Year of Publication2003
AuthorsHammer, BK, Bassler, BL
JournalMol Microbiol
Volume50
Issue1
Pagination101-4
Date Published2003 Oct
ISSN0950-382X
KeywordsBacterial Proteins, beta-Galactosidase, Biofilms, DNA Mutational Analysis, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genes, Regulator, Genes, Reporter, Luciferases, Operon, Polysaccharides, Repressor Proteins, Signal Transduction, Trans-Activators, Vibrio cholerae
Abstract

<p>Multiple quorum-sensing circuits function in parallel to control virulence and biofilm formation in Vibrio cholerae. In contrast to other bacterial pathogens that induce virulence factor production and/or biofilm formation at high cell density in the presence of quorum-sensing autoinducers, V. cholerae represses these behaviours at high cell density. Consistent with this, we show here that V. cholerae strains 'locked' in the regulatory state mimicking low cell density are enhanced for biofilm production whereas mutants 'locked' in the regulatory state mimicking high cell density are incapable of producing biofilms. The quorum-sensing cascade we have identified in V. cholerae regulates the transcription of genes involved in exopolysaccharide production (EPS), and variants that produce EPS and form biofilms arise at high frequency from non-EPS, non-biofilm producing strains. Our data show that spontaneous mutation of the transcriptional regulator hapR is responsible for this effect. Several toxigenic strains of V. cholerae possess a naturally occurring frameshift mutation in hapR. Thus, the distinct environments occupied by this aquatic pathogen presumably include niches where cell-cell communication is crucial, as well as ones where loss of quorum sensing via hapR mutation confers a selective advantage. Bacterial biofilms could represent a complex habitat where such differentiation occurs.</p>

DOI10.1046/j.1365-2958.2003.03688.x
Alternate JournalMol. Microbiol.
PubMed ID14507367