Title | Pushing, pulling, and squeezing our way to understanding mechanotransduction. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Siedlik, MJ, Varner, VD, Nelson, CM |
Journal | Methods |
Volume | 94 |
Pagination | 4-12 |
Date Published | 2016 Feb 01 |
ISSN | 1095-9130 |
Keywords | Animals, Biomechanical Phenomena, Cell Culture Techniques, Cells, Cultured, Epithelial Cells, Fluorescence Resonance Energy Transfer, Humans, Mechanotransduction, Cellular, Microscopy, Atomic Force |
Abstract | <p>Mechanotransduction is often described in the context of force-induced changes in molecular conformation, but molecular-scale mechanical stimuli arise in vivo in the context of complex, multicellular tissue structures. For this reason, we highlight and review experimental methods for investigating mechanotransduction across multiple length scales. We begin by discussing techniques that probe the response of individual molecules to applied force. We then move up in length scale to highlight techniques aimed at uncovering how cells transduce mechanical stimuli into biochemical activity. Finally, we discuss approaches for determining how these stimuli arise in multicellular structures. We expect that future work will combine techniques across these length scales to provide a more comprehensive understanding of mechanotransduction. </p> |
DOI | 10.1016/j.ymeth.2015.08.019 |
Alternate Journal | Methods |
PubMed ID | 26318086 |
PubMed Central ID | PMC4761538 |
Grant List | R21 HL110335 / HL / NHLBI NIH HHS / United States HL110335 / HL / NHLBI NIH HHS / United States R21 HL118532 / HL / NHLBI NIH HHS / United States R01 GM083997 / GM / NIGMS NIH HHS / United States R01 HL120142 / HL / NHLBI NIH HHS / United States HL118532 / HL / NHLBI NIH HHS / United States GM083997 / GM / NIGMS NIH HHS / United States HL120142 / HL / NHLBI NIH HHS / United States |