Pushing, pulling, and squeezing our way to understanding mechanotransduction.

TitlePushing, pulling, and squeezing our way to understanding mechanotransduction.
Publication TypeJournal Article
Year of Publication2016
AuthorsSiedlik, MJ, Varner, VD, Nelson, CM
JournalMethods
Volume94
Pagination4-12
Date Published2016 Feb 01
ISSN1095-9130
KeywordsAnimals, Biomechanical Phenomena, Cell Culture Techniques, Cells, Cultured, Epithelial Cells, Fluorescence Resonance Energy Transfer, Humans, Mechanotransduction, Cellular, Microscopy, Atomic Force
Abstract

<p>Mechanotransduction is often described in the context of force-induced changes in molecular conformation, but molecular-scale mechanical stimuli arise in vivo in the context of complex, multicellular tissue structures. For this reason, we highlight and review experimental methods for investigating mechanotransduction across multiple length scales. We begin by discussing techniques that probe the response of individual molecules to applied force. We then move up in length scale to highlight techniques aimed at uncovering how cells transduce mechanical stimuli into biochemical activity. Finally, we discuss approaches for determining how these stimuli arise in multicellular structures. We expect that future work will combine techniques across these length scales to provide a more comprehensive understanding of mechanotransduction. </p>

DOI10.1016/j.ymeth.2015.08.019
Alternate JournalMethods
PubMed ID26318086
PubMed Central IDPMC4761538
Grant ListR21 HL110335 / HL / NHLBI NIH HHS / United States
HL110335 / HL / NHLBI NIH HHS / United States
R21 HL118532 / HL / NHLBI NIH HHS / United States
R01 GM083997 / GM / NIGMS NIH HHS / United States
R01 HL120142 / HL / NHLBI NIH HHS / United States
HL118532 / HL / NHLBI NIH HHS / United States
GM083997 / GM / NIGMS NIH HHS / United States
HL120142 / HL / NHLBI NIH HHS / United States