Protocol for transgenerational learned pathogen avoidance behavior assays in . Author Rebecca Moore, Rachel Kaletsky, Coleen Murphy Publication Year 2021 Type Journal Article Abstract Animal experiences, including learned behaviors, can be passed down to several generations of progeny in a phenomenon known as transgenerational epigenetic inheritance. Yet, little is known regarding the molecular mechanisms regulating physiologically relevant transgenerational memories. Here, we present a method for in which worms learn to avoid the pathogen (PA14). Unlike previous protocols, this training paradigm, either using PA14 lawns or through exposure to a PA14 small RNA (P11), induces memory in four generations of progeny. For complete details on the use and execution of this protocol, please refer to Moore et al. (2019) and Kaletsky et al. (2020). Keywords Animals, Pseudomonas aeruginosa, Caenorhabditis elegans, RNA, Learning, Memory, Caenorhabditis elegans Proteins, Epigenesis, Genetic, Epigenomics, Avoidance Learning, Inheritance Patterns, Conditioning, Psychological, Wills Journal STAR Protoc Volume 2 Issue 1 Pages 100384 Date Published 2021 Mar 19 ISSN Number 2666-1667 DOI 10.1016/j.xpro.2021.100384 Alternate Journal STAR Protoc PMCID PMC7960678 PMID 33748786 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML