Protocol for transgenerational learned pathogen avoidance behavior assays in .

TitleProtocol for transgenerational learned pathogen avoidance behavior assays in .
Publication TypeJournal Article
Year of Publication2021
AuthorsMoore, RS, Kaletsky, R, Murphy, CT
JournalSTAR Protoc
Date Published2021 Mar 19
KeywordsAnimals, Avoidance Learning, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Conditioning, Psychological, Epigenesis, Genetic, Epigenomics, Inheritance Patterns, Learning, Memory, Pseudomonas aeruginosa, RNA, Wills

<p>Animal experiences, including learned behaviors, can be passed down to several generations of progeny in a phenomenon known as transgenerational epigenetic inheritance. Yet, little is known regarding the molecular mechanisms regulating physiologically relevant transgenerational memories. Here, we present a method for in which worms learn to avoid the pathogen (PA14). Unlike previous protocols, this training paradigm, either using PA14 lawns or through exposure to a PA14 small RNA (P11), induces memory in four generations of progeny. For complete details on the use and execution of this protocol, please refer to Moore et al. (2019) and Kaletsky et al. (2020).</p>

Alternate JournalSTAR Protoc
PubMed ID33748786
PubMed Central IDPMC7960678