Protocol for transgenerational learned pathogen avoidance behavior assays in .

Publication Year
2021

Type

Journal Article
Abstract

Animal experiences, including learned behaviors, can be passed down to several generations of progeny in a phenomenon known as transgenerational epigenetic inheritance. Yet, little is known regarding the molecular mechanisms regulating physiologically relevant transgenerational memories. Here, we present a method for in which worms learn to avoid the pathogen (PA14). Unlike previous protocols, this training paradigm, either using PA14 lawns or through exposure to a PA14 small RNA (P11), induces memory in four generations of progeny. For complete details on the use and execution of this protocol, please refer to Moore et al. (2019) and Kaletsky et al. (2020).

Journal
STAR Protoc
Volume
2
Issue
1
Pages
100384
Date Published
2021 Mar 19
ISSN Number
2666-1667
Alternate Journal
STAR Protoc
PMCID
PMC7960678
PMID
33748786