Proteomics of nucleocytoplasmic partitioning. Author Thao Nguyen, Nishant Pappireddi, Martin Wühr Publication Year 2019 Type Journal Article Abstract The partitioning of the proteome between nucleus and cytoplasm affects nearly every aspect of eukaryotic biology. Despite this central role, we still have a poor understanding of which proteins localize in the nucleus and how this varies in different cell types and conditions. Recent advances in quantitative proteomics and high-throughput imaging are starting to close this knowledge gap. Studies on protein interaction are beginning to reveal the spectrum of cargos of nuclear import and export receptors. We anticipate that it will soon be possible to predict each protein's nucleocytoplasmic localization based on its importin/exportin interactions and its estimated diffusion rate through the nuclear pore. This insight is likely to provide us with a fundamental understanding of how cells use nucleocytoplasmic partitioning to encode and relay information. Keywords Animals, Humans, Cell Nucleus, Proteomics, Proteome, Protein Transport, Active Transport, Cell Nucleus, Cytoplasm, Protein Interaction Maps, Karyopherins Journal Curr Opin Chem Biol Volume 48 Pages 55-63 Date Published 2019 Feb ISSN Number 1879-0402 DOI 10.1016/j.cbpa.2018.10.027 Alternate Journal Curr Opin Chem Biol PMCID PMC6382543 PMID 30472625 PubMedPubMed CentralGoogle ScholarBibTeXEndNote X3 XML