Proteomics of nucleocytoplasmic partitioning.

TitleProteomics of nucleocytoplasmic partitioning.
Publication TypeJournal Article
Year of Publication2019
AuthorsNguyen, T, Pappireddi, N, Wühr, M
JournalCurr Opin Chem Biol
Date Published2019 02
KeywordsActive Transport, Cell Nucleus, Animals, Cell Nucleus, Cytoplasm, Humans, Karyopherins, Protein Interaction Maps, Protein Transport, Proteome, Proteomics

<p>The partitioning of the proteome between nucleus and cytoplasm affects nearly every aspect of eukaryotic biology. Despite this central role, we still have a poor understanding of which proteins localize in the nucleus and how this varies in different cell types and conditions. Recent advances in quantitative proteomics and high-throughput imaging are starting to close this knowledge gap. Studies on protein interaction are beginning to reveal the spectrum of cargos of nuclear import and export receptors. We anticipate that it will soon be possible to predict each protein's nucleocytoplasmic localization based on its importin/exportin interactions and its estimated diffusion rate through the nuclear pore. This insight is likely to provide us with a fundamental understanding of how cells use nucleocytoplasmic partitioning to encode and relay information.</p>

Alternate JournalCurr Opin Chem Biol
PubMed ID30472625
PubMed Central IDPMC6382543
Grant ListR35 GM128813 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States