The Proteome of Preretinal Tissue in Proliferative Vitreoretinopathy.

TitleThe Proteome of Preretinal Tissue in Proliferative Vitreoretinopathy.
Publication TypeJournal Article
Year of Publication2021
AuthorsMiller, CG, Henderson, M, Mantopoulos, D, Leskov, I, Greco, T, Schwarzbauer, JE, Prenner, JL
JournalOphthalmic Surg Lasers Imaging Retina
Volume52
IssueS1
PaginationS5-S12
Date Published2021/07/31
ISSN2325-8179
KeywordsEpiretinal Membrane, Humans, Proteome, Retina, Retinal Detachment, Vitreoretinopathy, Proliferative, Vitreous Body
Abstract

BACKGROUND AND OBJECTIVE: Proliferative vitreoretinopathy (PVR) is the leading cause of retinal detachment repair failure. However, the molecular pathogenesis remains incompletely understood. Determining the proteome of PVR will help to identify novel therapeutic targets.

MATERIALS AND METHODS: Preretinal tissue samples, delaminated during surgery from six PVR cases and one idiopathic epiretinal membrane (ERM) were analyzed by mass spectrometry. Tandem mass spectra were extracted using the UniProt database, generating a list of 896 proteins, which were subjected to pathway set and fold-change (ERM vs PVR) analyses.

RESULTS: Two pathways were enriched in PVR: extracellular matrix (ECM) organization and extracellular structure organization. A fold-change analysis comparing mean total spectral counts from PVR to an ERM control identified fibronectin, the ECM glycoprotein, as the protein most significantly elevated in PVR compared to ERM.

CONCLUSION: These data identify pathwayskey to PVR progression, including thoseinvolved in cell-mediated ECM assembly and thus tractional force generation at the cellular level. .

DOI10.3928/23258160-20210518-02
Alternate JournalOphthalmic Surg Lasers Imaging Retina
PubMed ID34310239