Properties of repression condensates in living Ciona embryos.

TitleProperties of repression condensates in living Ciona embryos.
Publication TypeJournal Article
Year of Publication2021
AuthorsTreen, N, Shimobayashi, SF, Eeftens, J, Brangwynne, CP, Levine, M
JournalNat Commun
Volume12
Issue1
Pagination1561
Date Published2021 03 10
ISSN2041-1723
KeywordsAnimals, Ciona, Embryo, Nonmammalian, Gene Expression Regulation, Developmental, Homeodomain Proteins, Transcription Factors, Transcription, Genetic
Abstract

<p>Recent studies suggest that transcriptional activators and components of the pre-initiation complex (PIC) form higher order associations-clusters or condensates-at active loci. Considerably less is known about the distribution of repressor proteins responsible for gene silencing. Here, we develop an expression assay in living Ciona embryos that captures the liquid behavior of individual nucleoli undergoing dynamic fusion events. The assay is used to visualize puncta of Hes repressors, along with the Groucho/TLE corepressor. We observe that Hes.a/Gro puncta have the properties of viscous liquid droplets that undergo limited fusion events due to association with DNA. Hes.a mutants that are unable to bind DNA display hallmarks of liquid-liquid phase separation, including dynamic fusions of individual condensates to produce large droplets. We propose that the DNA template serves as a scaffold for the formation of Hes condensates, but limits the spread of transcriptional repressors to unwanted regions of the genome.</p>

DOI10.1038/s41467-021-21606-5
Alternate JournalNat Commun
PubMed ID33692345
PubMed Central IDPMC7946874
Grant ListR01 NS076542 / NS / NINDS NIH HHS / United States
U01 DA040601 / DA / NIDA NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States