Title | Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Cao, WXi, Kabelitz, S, Gupta, M, Yeung, E, Lin, S, Rammelt, C, Ihling, C, Pekovic, F, Low, TCH, Siddiqui, NU, Cheng, MHK, Angers, S, Smibert, CA, Wühr, M, Wahle, E, Lipshitz, HD |
Journal | Cell Rep |
Volume | 31 |
Issue | 12 |
Pagination | 107783 |
Date Published | 2020 06 23 |
ISSN | 2211-1247 |
Abstract | <p>In animal embryos, the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents more than half of the protein-coding capacity of Drosophila melanogaster's genome, and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). Although the ubiquitin-proteasome system is necessary for clearance of these repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL, and ME31B for degradation early in the MZT and the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG abrogates F-box protein interaction and confers immunity to degradation. Persistent SMG downregulates zygotic re-expression of mRNAs whose maternal contribution is degraded by SMG. Thus, clearance of SMG permits an orderly MZT.</p> |
DOI | 10.1016/j.celrep.2020.107783 |
Alternate Journal | Cell Rep |
PubMed ID | 32579915 |
PubMed Central ID | PMC7372737 |
Grant List | R35 GM128813 / GM / NIGMS NIH HHS / United States T32 GM007388 / GM / NIGMS NIH HHS / United States PJT-159702 / / CIHR / Canada |