Positional information, positional error, and readout precision in morphogenesis: a mathematical framework.

TitlePositional information, positional error, and readout precision in morphogenesis: a mathematical framework.
Publication TypeJournal Article
Year of Publication2015
AuthorsTkačik, G, Dubuis, JO, Petkova, MD, Gregor, T
JournalGenetics
Volume199
Issue1
Pagination39-59
Date Published2015 Jan
ISSN1943-2631
KeywordsAnimals, Drosophila, Gene Expression Regulation, Developmental, Models, Genetic, Morphogenesis
Abstract

<p>The concept of positional information is central to our understanding of how cells determine their location in a multicellular structure and thereby their developmental fates. Nevertheless, positional information has neither been defined mathematically nor quantified in a principled way. Here we provide an information-theoretic definition in the context of developmental gene expression patterns and examine the features of expression patterns that affect positional information quantitatively. We connect positional information with the concept of positional error and develop tools to directly measure information and error from experimental data. We illustrate our framework for the case of gap gene expression patterns in the early Drosophila embryo and show how information that is distributed among only four genes is sufficient to determine developmental fates with nearly single-cell resolution. Our approach can be generalized to a variety of different model systems; procedures and examples are discussed in detail. </p>

DOI10.1534/genetics.114.171850
Alternate JournalGenetics
PubMed ID25361898
PubMed Central IDPMC4286692
Grant ListR01 GM097275 / GM / NIGMS NIH HHS / United States