Persister formation in Escherichia coli can be inhibited by treatment with nitric oxide.

TitlePersister formation in Escherichia coli can be inhibited by treatment with nitric oxide.
Publication TypeJournal Article
Year of Publication2016
AuthorsOrman, MA, Brynildsen, MP
JournalFree Radic Biol Med
Volume93
Pagination145-54
Date Published2016 Apr
ISSN1873-4596
KeywordsAnti-Bacterial Agents, Biofilms, Drug Resistance, Bacterial, Escherichia coli, Humans, Microbial Sensitivity Tests, Nitric Oxide, Protein Biosynthesis, Proteolysis, RNA Stability
Abstract

<p>Bacterial persisters are phenotypic variants that survive extraordinary concentrations of antibiotics, and are thought to underlie the propensity of biofilm infections to relapse. Unfortunately many aspects of persister physiology remain ill-defined, which prevents progress toward eradicating the phenotype. Recently, we identified respiration within non-growing Escherichia coli populations as a potential target for the elimination type I persisters, which are those that arise from passage through stationary phase. Here we discovered that nitric oxide (NO) treatment at the onset of stationary phase significantly reduced type I persister formation through its ability to inhibit respiration. NO decreased protein and RNA degradation in stationary phase cells, and produced populations that were more fit for protein synthesis and growth resumption upon introduction into fresh media than untreated controls. Overall, this data shows that NO, which is a therapeutically-relevant compound, has the potential to decrease the incidence of recurrent infections from persisters. </p>

DOI10.1016/j.freeradbiomed.2016.02.003
Alternate JournalFree Radic. Biol. Med.
PubMed ID26849946
PubMed Central IDPMC4898466
Grant ListR21 AI115075 / AI / NIAID NIH HHS / United States
R21AI115075 / AI / NIAID NIH HHS / United States