Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila.

TitlePaip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila.
Publication TypeJournal Article
Year of Publication2018
AuthorsKachaev, ZM, Lebedeva, LA, Kozlov, EN, Toropygin, IY, Schedl, P, Shidlovskii, YV
JournalCell Cycle
Date Published2018
KeywordsAnimals, Carrier Proteins, Cell Line, Chromatin, Drosophila melanogaster, Drosophila Proteins, Embryo, Nonmammalian, Male, Poly(A)-Binding Proteins, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Testis

<p>Paip2 (Poly(A)-binding protein - interacting protein 2) is a conserved metazoan-specific protein that has been implicated in regulating the translation and stability of mRNAs. However, we have found that Paip2 is not restricted to the cytoplasm but is also found in the nucleus in Drosophila embryos, salivary glands, testes, and tissue culture cells. Nuclear Paip2 is associated with chromatin, and in chromatin immunoprecipitation experiments it maps to the promoter regions of active genes. However, this chromatin association is indirect, as it is RNA-dependent. Thus, Paip2 is one more item in the growing list of translation factors that are recruited to mRNAs co-transcriptionally.</p>

Alternate JournalCell Cycle
PubMed ID29995569
PubMed Central IDPMC6133343
Grant ListR01 GM110015 / GM / NIGMS NIH HHS / United States
R35 GM126975 / GM / NIGMS NIH HHS / United States