Title | The p53 protein does not facilitate adenovirus type 5 replication in normal human cells. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Chahal, JS, Flint, SJ |
Journal | J Virol |
Volume | 87 |
Issue | 10 |
Pagination | 6044-6 |
Date Published | 2013 May |
ISSN | 1098-5514 |
Keywords | Adenoviruses, Human, Cells, Cultured, Epithelial Cells, Gene Knockdown Techniques, Host-Pathogen Interactions, Humans, RNA Interference, Tumor Suppressor Protein p53, Virus Replication |
Abstract | <p>Although several adenovirus type 5 (Ad5) proteins prevent deleterious consequences of activation of p53, it has been reported that viral replication proceeds more efficiently when human tumor cells produce wild-type compared to mutant p53. We have now exploited RNA interference and lentiviral vectors to achieve essentially complete knockdown of p53 in normal human cells: no effects on the kinetics or efficiency of viral gene expression or production of infectious particles were observed.</p> |
DOI | 10.1128/JVI.00129-13 |
Alternate Journal | J Virol |
PubMed ID | 23487462 |
PubMed Central ID | PMC3648133 |
Grant List | R56 AI091785 / AI / NIAID NIH HHS / United States R56AI1691785 / AI / NIAID NIH HHS / United States |