| Title | The p53 protein does not facilitate adenovirus type 5 replication in normal human cells. |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Chahal, JS, Flint, SJ |
| Journal | J Virol |
| Volume | 87 |
| Issue | 10 |
| Pagination | 6044-6 |
| Date Published | 2013 May |
| ISSN | 1098-5514 |
| Keywords | Adenoviruses, Human, Cells, Cultured, Epithelial Cells, Gene Knockdown Techniques, Host-Pathogen Interactions, Humans, RNA Interference, Tumor Suppressor Protein p53, Virus Replication |
| Abstract | <p>Although several adenovirus type 5 (Ad5) proteins prevent deleterious consequences of activation of p53, it has been reported that viral replication proceeds more efficiently when human tumor cells produce wild-type compared to mutant p53. We have now exploited RNA interference and lentiviral vectors to achieve essentially complete knockdown of p53 in normal human cells: no effects on the kinetics or efficiency of viral gene expression or production of infectious particles were observed.</p> |
| DOI | 10.1128/JVI.00129-13 |
| Alternate Journal | J Virol |
| PubMed ID | 23487462 |
| PubMed Central ID | PMC3648133 |
| Grant List | R56 AI091785 / AI / NIAID NIH HHS / United States R56AI1691785 / AI / NIAID NIH HHS / United States |
