Overriding native cell coordination enhances external programming of collective cell migration.

TitleOverriding native cell coordination enhances external programming of collective cell migration.
Publication TypeJournal Article
Year of Publication2021
AuthorsShim, G, Devenport, D, Cohen, DJ
JournalProc Natl Acad Sci U S A
Volume118
Issue29
Date Published2021/07/20
ISSN1091-6490
Abstract

As collective cell migration is essential in biological processes spanning development, healing, and cancer progression, methods to externally program cell migration are of great value. However, problems can arise if the external commands compete with strong, preexisting collective behaviors in the tissue or system. We investigate this problem by applying a potent external migratory cue-electrical stimulation and electrotaxis-to primary mouse skin monolayers where we can tune cell-cell adhesion strength to modulate endogenous collectivity. Monolayers with high cell-cell adhesion showed strong natural coordination and resisted electrotactic control, with this conflict actively damaging the leading edge of the tissue. However, reducing preexisting coordination in the tissue by specifically inhibiting E-cadherin-dependent cell-cell adhesion, either by disrupting the formation of cell-cell junctions with E-cadherin-specific antibodies or rapidly dismantling E-cadherin junctions with calcium chelators, significantly improved controllability. Finally, we applied this paradigm of weakening existing coordination to improve control and demonstrate accelerated wound closure in vitro. These results are in keeping with those from diverse, noncellular systems and confirm that endogenous collectivity should be considered as a key quantitative design variable when optimizing external control of collective migration.

DOI10.1073/pnas.2101352118
Alternate JournalProc Natl Acad Sci U S A
PubMed ID34272284
PubMed Central IDPMC8307614
Grant ListR35 GM133574 / GM / NIGMS NIH HHS / United States