Outer Membrane Protein Insertion by the β-barrel Assembly Machine.

TitleOuter Membrane Protein Insertion by the β-barrel Assembly Machine.
Publication TypeJournal Article
Year of Publication2019
AuthorsRicci, DP, Silhavy, TJ
JournalEcoSal Plus
Date Published2019 03
KeywordsBacterial Outer Membrane Proteins, Escherichia coli, Escherichia coli Proteins, Gram-Negative Bacteria, Protein Folding, Protein Transport

<p>Like all outer membrane (OM) constituents, integral OM β-barrel proteins in Gram-negative bacteria are synthesized in the cytoplasm and trafficked to the OM, where they are locally assembled into the growing OM by the ubiquitous β-barrel assembly machine (Bam). While the identities and structures of all essential and accessory Bam components have been determined, the basic mechanism of Bam-assisted OM protein integration remains elusive. Here we review mechanistic analyses of OM β-barrel protein folding and Bam dynamics and summarize recent insights that inform a general model for OM protein recognition and assembly by the Bam complex.</p>

Alternate JournalEcoSal Plus
PubMed ID30869065
PubMed Central IDPMC6419762
Grant ListR35 GM118024 / GM / NIGMS NIH HHS / United States