Title | Outer Membrane Biogenesis. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Konovalova, A, Kahne, DE, Silhavy, TJ |
Journal | Annu Rev Microbiol |
Volume | 71 |
Pagination | 539-556 |
Date Published | 2017 Sep 08 |
ISSN | 1545-3251 |
Keywords | Bacterial Outer Membrane Proteins, Gram-Negative Bacteria, Lipid Bilayers, Lipopolysaccharides, Membranes, Organelle Biogenesis |
Abstract | <p>The hallmark of gram-negative bacteria and organelles such as mitochondria and chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the outer membrane is a unique asymmetric lipid bilayer with lipopolysaccharide in the outer leaflet. Integral transmembrane proteins assume a β-barrel structure, and their assembly is catalyzed by the heteropentameric Bam complex containing the outer membrane protein BamA and four lipoproteins, BamB-E. How the Bam complex assembles a great diversity of outer membrane proteins into a membrane without an obvious energy source is a particularly challenging problem, because folding intermediates are predicted to be unstable in either an aqueous or a hydrophobic environment. Two models have been put forward: the budding model, based largely on structural data, and the BamA assisted model, based on genetic and biochemical studies. Here we offer a critical discussion of the pros and cons of each.</p> |
DOI | 10.1146/annurev-micro-090816-093754 |
Alternate Journal | Annu Rev Microbiol |
PubMed ID | 28886680 |
PubMed Central ID | PMC5778897 |
Grant List | R35 GM118024 / GM / NIGMS NIH HHS / United States |