Orchestration of protein acetylation as a toggle for cellular defense and virus replication.

TitleOrchestration of protein acetylation as a toggle for cellular defense and virus replication.
Publication TypeJournal Article
Year of Publication2018
AuthorsMurray, LA, Sheng, X, Cristea, IM
JournalNat Commun
Volume9
Issue1
Pagination4967
Date Published2018 11 23
ISSN2041-1723
Abstract

Emerging evidence highlights protein acetylation, a prevalent lysine posttranslational modification, as a regulatory mechanism and promising therapeutic target in human viral infections. However, how infections dynamically alter global cellular acetylation or whether viral proteins are acetylated remains virtually unexplored. Here, we establish acetylation as a highly-regulated molecular toggle of protein function integral to the herpesvirus human cytomegalovirus (HCMV) replication. We offer temporal resolution of cellular and viral acetylations. By interrogating dynamic protein acetylation with both protein abundance and subcellular localization, we discover finely tuned spatial acetylations across infection time. We determine that lamin acetylation at the nuclear periphery protects against virus production by inhibiting capsid nuclear egress. Further studies within infectious viral particles identify numerous acetylations, including on the viral transcriptional activator pUL26, which we show represses virus production. Altogether, this study provides specific insights into functions of cellular and viral protein acetylations and a valuable resource of dynamic acetylation events.

DOI10.1038/s41467-018-07179-w
Alternate JournalNat Commun
PubMed ID30470744
PubMed Central IDPMC6251895
Grant ListR01 GM114141 / GM / NIGMS NIH HHS / United States
T32 GM007388 / GM / NIGMS NIH HHS / United States