Optogenetic regulation of engineered cellular metabolism for microbial chemical production.

TitleOptogenetic regulation of engineered cellular metabolism for microbial chemical production.
Publication TypeJournal Article
Year of Publication2018
AuthorsZhao, EM, Zhang, Y, Mehl, J, Park, H, Lalwani, MA, Toettcher, JE, Avalos, JL
JournalNature
Volume555
Issue7698
Pagination683-687
Date Published2018 Mar 29
ISSN1476-4687
Abstract

The optimization of engineered metabolic pathways requires careful control over the levels and timing of metabolic enzyme expression. Optogenetic tools are ideal for achieving such precise control, as light can be applied and removed instantly without complex media changes. Here we show that light-controlled transcription can be used to enhance the biosynthesis of valuable products in engineered Saccharomyces cerevisiae. We introduce new optogenetic circuits to shift cells from a light-induced growth phase to a darkness-induced production phase, which allows us to control fermentation with only light. Furthermore, optogenetic control of engineered pathways enables a new mode of bioreactor operation using periodic light pulses to tune enzyme expression during the production phase of fermentation to increase yields. Using these advances, we control the mitochondrial isobutanol pathway to produce up to 8.49 ± 0.31 g lof isobutanol and 2.38 ± 0.06 g lof 2-methyl-1-butanol micro-aerobically from glucose. These results make a compelling case for the application of optogenetics to metabolic engineering for the production of valuable products.

DOI10.1038/nature26141
Alternate JournalNature
PubMed ID29562237
PubMed Central IDPMC5876151
Grant ListDP2 EB024247 / EB / NIBIB NIH HHS / United States