Optogenetic Reconstitution for Determining the Form and Function of Membraneless Organelles.

TitleOptogenetic Reconstitution for Determining the Form and Function of Membraneless Organelles.
Publication TypeJournal Article
Year of Publication2018
AuthorsDine, E, Toettcher, JE
JournalBiochemistry
Date Published2018 Jan 26
ISSN1520-4995
Abstract

It has recently become clear that large-scale macromolecular self-assembly is a rule, rather than an exception, of intracellular organization. A growing number of proteins and RNAs have been shown to self-assemble into micrometer-scale clusters that exhibit either liquid-like or gel-like properties. Given their proposed roles in intracellular regulation, embryo development, and human disease, it is becoming increasingly important to understand how these membraneless organelles form and to map their functional consequences for the cell. Recently developed optogenetic systems make it possible to acutely control cluster assembly and disassembly in live cells, driving the separation of proteins of interest into liquid droplets, hydrogels, or solid aggregates. Here we propose that these approaches, as well as their evolution into the next generation of optogenetic biophysical tools, will allow biologists to determine how the self-assembly of membraneless organelles modulates diverse biochemical processes.

DOI10.1021/acs.biochem.7b01173
Alternate JournalBiochemistry
PubMed ID29373016
Grant ListDP2 EB024247 / EB / NIBIB NIH HHS / United States