Optogenetic dissection of descending behavioral control in .

TitleOptogenetic dissection of descending behavioral control in .
Publication TypeJournal Article
Year of Publication2018
AuthorsCande, J, Namiki, S, Qiu, J, Korff, W, Card, GM, Shaevitz, JW, Stern, DL, Berman, GJ
JournalElife
Volume7
Date Published2018 06 26
ISSN2050-084X
KeywordsAnimals, Behavior, Animal, Biological Assay, Brain, Brain Mapping, Drosophila melanogaster, Drosophila Proteins, Efferent Pathways, Genes, Reporter, Locomotion, Neurons, Optogenetics, Spatial Behavior, Transcription Factors
Abstract

<p>In most animals, the brain makes behavioral decisions that are transmitted by descending neurons to the nerve cord circuitry that produces behaviors. In insects, only a few descending neurons have been associated with specific behaviors. To explore how descending neurons control an insect's movements, we developed a novel method to systematically assay the behavioral effects of activating individual neurons on freely behaving terrestrial . We calculated a two-dimensional representation of the entire behavior space explored by these flies, and we associated descending neurons with specific behaviors by identifying regions of this space that were visited with increased frequency during optogenetic activation. Applying this approach across a large collection of descending neurons, we found that (1) activation of most of the descending neurons drove stereotyped behaviors, (2) in many cases multiple descending neurons activated similar behaviors, and (3) optogenetically activated behaviors were often dependent on the behavioral state prior to activation.</p>

DOI10.7554/eLife.34275
Alternate JournalElife
PubMed ID29943729
PubMed Central IDPMC6031430
Grant ListR01 GM098090 / GM / NIGMS NIH HHS / United States
GM09809 / NH / NIH HHS / United States
/ HHMI / Howard Hughes Medical Institute / United States